Gurjit Kaur Bhatti
Post Doc Fellow, Panjab University
As a Biochemist doctorate along with post graduate in dietetics and food management have passion in challenging research projects based on nutrigenetics. Worked on many genetic association studies in coronary artery disease and diabetic patients. Many research publication in toxigenomics area too.Working on nanoformulations of statins and drug delivery systems to improve the bioavalabilty.
Genetic polymorphisms in glutathione S-transferase (GSTs) genes may modulate the risk of cardiovascular diseases. The objective of present study was to investigate the potential association between the polymorphisms of GSTM1/T1 and P1 genes and their influence on diverse clinical parameters and oxidative stress biomarkers in coronary artery disease (CAD) patients in Asian Indians. Present study includes 562 angiographically confirmed CAD patients and 564 healthy control subjects from north Indian population. Anthropometric and clinical measurements were done in all the participants. The oxidative stress biomarkers including malondialdehyde (MDA) and total antioxidant capacity (TAC) were also measured. The genotyping of GSTM1/T1 and P1 genes was carried out using multiplex-PCR and PCR-RFLP methods. The CAD patients exhibit significantly high values of waist circumference (WC), waist to hip ratio (WHR), Body fat(%), glucose, triglycerides and low-density lipoprotein (LDL), and reduced HDL levels were observed in CAD patients compared to control subjects (p<0.001). MDA levels were significantly enhanced and TAC was reduced in CAD patients compared to controls (p<0.001). However, no significant difference in BMI and total cholesterol levels was observed in CAD patients and control subjects. The frequencies of the GSTM1 and GSTM1/T1 null genotypes in the CAD patients were significantly higher than the control subjects. In contrast, GSTT1(-) genotype frequencies were significantly lower in CAD patients than the controls. Logistic regression analysis of the data revealed null genotype of GSTM1 and GG genotype of GSTP1 (313A/G) gene were associated with 2-fold enhanced risk of developing CAD whereas GSTT1(-) plays a defensive role against CAD development in north Indians. Upon stratification of data according to the genotypes of GSTM1/T1 and P1 genes, we did not find significant difference amongst the various metabolic traits in CAD patients and controls. Our results suggest that oxidative damage induced by lipid peroxidation with reduced antioxidant capacity and genetic variants in GST genes (GSTM1/T1 and P1) may modify the risk of CAD development in Asian Indian population.