Sri Guru Gobind Singh College, Chandigarh India
Title: Association of BsmI, TaqI and FokI polymorphisms in Vitamin D receptor gene with the risk of coronary artery disease in Asian Indians
Bhatti is an Assistant Professor having teaching and research experience of >10 years. Recently he finished his post doc fellowship at Texas Tech University Health Sciences Center, Lubbock TX USA under the scheme of Raman Fellowship funded by University Grants Commission Govt. of India. He identified a link between the type 2 diabetes and development of Alzheimer’s dises using a genetic model of type 2 diabetes, TallyHO. He is actively engaged in investigating the genes associated with the risk of developing type 2 diabetes and coronary artery disease (CAD) in Asian Indians. He has published 25 research papers in reputed international journals and presented his findings in various national and international conferences. He is an expertise in the area of gene based personalized medicine. He has identified various life style related risk factors and susceptibility genes in diabetes, CAD and metabolic syndrome.
Statement of the Problem: Coronary artery disease (CAD) is a multifactorial syndrome that is deemed to be an outcome of the association between genetic and environmental factors. Vitamin D receptor (VDR) is a probable candidate gene for the development of CAD. The purpose of present study was planned to examine the role of single nucleotide polymorphisms (SNPs) in VDR gene and CAD risk in Asian Indians. Methodology: A total of 410 CAD patients and 414 controls were included. Three SNPs in VDR gene (BsmI, TaqI and FokI) were examined using PCR-RFLP method. In addition, the anthropometric and biochemical characteristics were done in all the subjects. Findings: The CAD patients shows pronounced abdominal adiposity reflected by their significantly higher waist circumference, waist to hip ratio, even at the normal BMI values suggested for Asian Indians. Dyslipidemia, represented by high levels of TC and TG, and reduced HDL was an established risk factor for development of CAD. The genotyping data revealed that BsmI-bb genotype might be associated with 2.2-fold increased risk (OR=2.19; 95% C.I. = 1.48-3.19; p=<0.001) whereas FokI-TT homozygotes had a 3.5 fold increased risk for the development of CAD (OR = 3.53; 95% C.I. = 2.33-5.36; p=<0.001). Furthermore, no significant relationship of TaqI polymorphism with CAD risk was observed (OR; 1.35, 95% C.I.; 0.91-1.99, p = 0.161). Conclusion & Significance: The present study demonstrated a significant association of BsmI and FokI variants of VDR gene with augmented possibility of CAD development in Asian Indian population. The conventional risk factors such as age, abdominal obesity and dyslipidemia were independently linked with the amplified risk of CAD. However, metabolic characteristics are not very affected by VDR gene polymorphisms in Asian Indians. Finding variations in the susceptibility genes that makes people more susceptible to diseases help us in improved therapeutic strategies.